Journal: PLOS Pathogens
Article Title: Secreted LysM proteins are required for niche competition and full virulence in Pseudomonas savastanoi during host plant infection
doi: 10.1371/journal.ppat.1013121
Figure Lengend Snippet: (A) Twitching motility on 1%-agar KB medium overlaid on slides by the wild-type strain Psv NCPPB 3335 (Psv) and the Δ lysM mutants. Microscopy images were taken after 24h of incubation at 25°C with Nikon Eclipse E800 light microscope (20x). The experiment was repeated three times with two repetitions per trial, and a representative image is shown per strain. (B-C) Swimming motility on 0.3%-agar KB medium. (B) Representative images of swimming halos formed by the wild-type strain Psv and the Δ lysM mutants after three days of incubation at 25°C. (C) Swimming halos are represented as the covered area (cm 2 ). Data are presented as means and standard deviation (SD) from three independent experiments, with at least four replicas per assay. Asterisks indicate statistically significant differences (ANOVA test; p < 0.0001), and “ns” indicates no significant differences between samples.
Article Snippet: After incubation, the twitching areas were examined using a Nikon Eclipse E800 light microscope with a 20x objective lens.
Techniques: Microscopy, Incubation, Light Microscopy, Standard Deviation